Volume : 11, Issue : 2 , Article : 4

Double-timed artificial insemination along with gonadorelin acetate injection improved the fertility in repeat breeder cows

  1. Mir Md. Iqbal Hasan , Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh and Department of Physiology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh
  2. Moinul Hasan , Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  3. Rupam Chandra Mohanta , Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  4. Md. Abu Haris Miah , Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  5. Marzia Rahman , Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  6. Md. Siddiqur Rahman , Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  7. Nasrin Sultana Juyena * , Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

* Corresponding Author

Abstract

Repeat breeding syndrome (RBS)is a multifactorial disorder in dairy cows, which cause a serious economic loss. The study was intended to know the effects of single-timed artificial insemination (SAI) and double-timed artificial insemination (DAI) with and without injection of gonadorelin acetate (GnRH analogue) for the improvement of fertility in repeat breeder (RB) cows. A total of 120 RB cows were selected in this study. Firstly, all cows were deworming and then divided into equal six groups namely; Group–ST1 (SAI), Group–ST2 (SAI with single injection of GnRH), Group–ST3 (SAI with double injection of GnRH), Group–DT1 (DAI), Group–DT2 (DAI with single injection of GnRH) and Group–DT3 (DAI with double injection of GnRH). The corpus luteum (CL) diameter and serum progesterone (P4) and estrogen (E2) hormone were estimated at day 18 post AI of the cows. Pregnancy rate was recorded at day 45–90 post AI. Diameter of CL and serum P4 were significantly (P<0.05) higher and serum E2 was significantly (P<0.05) lower in the RB cows with double injection of GnRH than others. Pregnancy rate was significantly (P<0.05) higher jointly in Group–DT2 and DT3 than other groups. Pregnancy was significantly (P<0.05) higher in DAI compared to SAI irrespective of GnRH injection strategies. It was also significantly (P<0.05) higher in AI with double injection of GnRH than AI without injection of GnRH irrespective of insemination strategies. Results confirm that double injection of GnRH and DAI improve the fertility in RB cows and the study helps Veterinarians and researchers to describe the insemination strategies for the prevention of RBS in dairy cows.

Keywords : GnRH injection, insemination strategies, ovarian and hormonal parameters, pregnancy rate, repeat estrus cows

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